Table 1. Description of bacteria.
Abbr. | Name | Description | Gram staining* |
---|---|---|---|
none | control | Mock infection | N/A |
Rv | MTB H37Rv | A common laboratory strain of MTB | acid-fast |
Rv+ | heat-inactivated MTB H37Rv | Dead MTB H37Rv | acid-fast |
GC | MTB GC1237 | More virulent strain of MTB | acid-fast |
BCG | bacillus Calmette-Guérin | Vaccine (attenuated M. bovis) | acid-fast |
Smeg | Mycobacterium smegmatis | Non-pathogenic mycobacterium | acid-fast |
Yers | Yersinia pseudotuberculosis | Facultative intracellular pathogen | Negative |
Salm | Salmonella typhimurium | Facultative intracellular pathogen | Negative |
Staph | Staphylococcus epidermidis | Extracellular pathogen | Positive |
*Mycobacteria are unable to be gram stained due to the low permeability of their cell walls. They are more closely related evolutionarily to gram-positive bacteria than gram-negative. However, their thick cells walls share features of gram-negative bacteria, e.g. a "pseudoperiplasm" similar to the gram-negative periplasm117.