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. 2015 Nov 20;5:16842. doi: 10.1038/srep16842

Figure 2. Characteristics of cardiovascular cell sheet constructs.

Figure 2

(A,B) Cross-sections of stacked sheets with or without 0.5 mg/cm2 of GHMs (φ 20–32 μm) inserted between. HE staining after 7-day culture. Scale bars, 50 μm. Magnifications, x200. (A) 5-Control: 5-sheet constructs without GHMs. (B) 5-GHM: 5-sheets with GHMs. (C,D) Thickness of constructs and viable cell area (HE-positive area) after 7-day culture. Mean ± s.e.m. **P < 0.01, *P < 0.05 (Mann-Whitney U-test, n = 5). (E,F) Immunostaining for cTnT (brown) after 7-day culture. 5-control (E) and 5-GHM (F) constructs. Scale bars, 50 μm. Magnifications, x200. (G) Quantitative evaluation of cTnT-positive and CD31-positive area. Mean ± s.e.m. **P < 0.01 (Mann-Whitney U test, cTnT, n = 9; CD31, n = 6).