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. 2015 Nov 20;5:16842. doi: 10.1038/srep16842

Figure 4. Efficient generation of thick viable cardiac cell structures with GHM method.

Figure 4

Thick GHM constructs with 10–15 cardiovascular cell sheets in vitro after 7-day culture. (A,B) HE staining. Note that construct thickness reaches more than 1 mm in 15-GHM. (C) cTnT (CMs; red), vWF (ECs; green) and DAPI (blue) staining. cTnT-negative/vWF-negative/DAPI-positive cells can be estimated as MCs. Inset: clear viable CM layers (red) include ECs (green). Scale bars, 100 μm. All photos are taken with x100 magnification.