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. 2015 Nov 20;5:16267. doi: 10.1038/srep16267

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(A) Schematic representation of PA oligomers, with each LF-binding site consisting of 3 subsites located on adjacent PA monomers. While a heptamer is shown, the same interactions occur within octamers. (B) Screening workflow to select improved intermolecular complementing PA variants. Unmethylated expression plasmids were isolated from dam⁻ and dcm⁻ E. coli SCS110, because only unmethylated plasmids are able to transform B. anthracis. BH480 transformants were grown and supernates assayed for protein expression and activity.