Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 20;5:16258. doi: 10.1038/srep16258

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Representative fluorescent images of MitoSOX^™-labeled ECs and (B) subsequent quantification (bar graph; n ≥ 30 cells) indicate that addition of 20-fold higher dose (100 μM) of free NTG to L-NIO-treated ECs significantly increases mitochondrial superoxide formation while similar increase in NTG-NL dose (0.07 μM to 1.4 μM) produces no effect. Scale bar: 100 μm. ***p < 0.001; ns, no significance. (C) The high dose (100 μM) of free NTG fails to suppress U937 monocytic cell adhesion to L-NIO-treated ECs (n = 10 fields of view). ***p < 0.001; ns, no significance. (D) Unlike free NTG, NTG-NL continues to exert potent anti-inflammatory effects on L-NIO-treated ECs even at a 20-fold higher dose (n = 10 fields of view) with all NTG-NL doses demonstrating similar inhibition of U937 adhesion. Therapeutic dose is indicated in bold. ***p < 0.001; ns, no significance. Data are expressed as mean ± SEM.