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. 2015 Nov 20;5:16459. doi: 10.1038/srep16459

Figure 5. The effect of HBV spliced variants 1, 2 and 3 on IFN signaling.

Figure 5

The cDNAs reverse-transcripted from the total RNAs extracted from the cells transfected with indicated plasmids were analyzed by PCR with the SP primer followed by agarose gel electrophoresis (a). The expression of proteins (*most likely to be the HBSP) in Huh7 cells transfected with indicated plasmids were analyzed by immunoblotting with anti-TP (b). Immunofluorescence assay was performed to detect the expression and cellular distribution of proteins containing TP domain in pSP1-transfected Huh7 cells using anti-TP Abs (c). Huh7 cells transfected with the indicated plasmids were mock- or IFN-α–treated for 1 hour and then harvested for immunoblotting (d), or for 12 h and then harvested for luciferase reporter assay (e,f). The data was analyzed using Student’s t-tests.