Figure 3. Treatment with ASC-CM did not affect expression of endogenous mouse wild type SOD1 and mutated human SOD1 transgene in spinal cord.

Wild type control (WT) and SOD1^G93A mice (vehicle and ASC-CM) spinal cords were prepared as detailed in materials and methods. Levels of actin were unchanged and used as an internal loading control for endogenous mouse wild type SOD1 (mSOD1^WT). There was no difference in mSOD1^WT levels, which was the loading control for mutated human SOD1 (hSOD1^G93A). After 7 days of ASC-CM treatment, there was no change in expression of either endogenous mSOD1^WT or hSOD1^G93A mice in symptomatic SOD1^G93A mice. n = 3 per group.