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Relative thermal stabilities of antithrombin P14 variants. Melting temperatures of the indicated antithrombin variants were measured by monitoring protein fluorescence changes over a temperature range encompassing the melting transition and fitting the melting curves by the van't Hoff equation as described under “Experimental Procedures.” Shown are the differences in the melting temperatures measured for the mutants (tabulated in Table 4) from that of wild type.
