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Nucleoplasmic PRC1 is monomeric. a, schematic depiction of the immobilization of YFP-PRC1 proteins on the surface by biotinylated anti-GFP antibody. The YFP-PRC1 complexes extracted from Cbx2−/−/Y-Cbx2, Cbx7−/−/Y-Cbx7, Ring1b−/−/Y-Ring1b, and Mel18−/−/Y-Mel18 mES cells were pulled down by biotinylated anti-GFP antibody via interaction with NeutrAvidin. b, percentage of fluorescence photobleaching steps of YFP-Cbx2, YFP-Cbx7, YFP-Ring1b, and YFP-Mel18 is shown. Results are means ± S.D.
