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. 2015 Sep 22;290(47):28107–28119. doi: 10.1074/jbc.M115.662197

FIGURE 6.

FIGURE 6.

Flt1 is a direct downstream target gene of Etv2. A, gene expression profile of Flk1, Flt1, and Vegfa following overexpression of Etv2 using iHA-Etv2 EBs on day 4. Flk1 is up-regulated 5-fold after 6 h of stimulation and 2-fold after 24 h of stimulation. Flt1 is up-regulated ∼2-fold after 6 h of stimulation and 6-fold after 24 h. *, p < 0.05. B, schematic of the ChIP assays using the Flt1 promoter. CRI is located in the 0.3-kb region, whereas CRII is located in the 3-kb region. C, Etv2 interacts with both CRI and CRII of the Flt1 promoter, as revealed using iHA-Etv2 EBs on day 4 and ChIP assays. *, p < 0.05. D, schematic of the 0.7-kb Flt1 promoter in the CRI region, which harbors six Ets motifs. WT, wild-type promoter; MUT, the mutated promoter with all six Ets motifs mutated; Luc, luciferase. E, Etv2 transactivates the 0.7-kb Flt1 reporter in a dose-dependent manner ∼15-fold in 293T cells. The transactivation is reduced to 4-fold when all Ets motifs were mutated. *, p < 0.05.