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. 2015 Oct 15;290(47):28189–28199. doi: 10.1074/jbc.M115.669499

FIGURE 3.

FIGURE 3.

Effect of AGE-BSA on the expression of autophagy related protein beclin-1, LC3, and p62 in hFOB 1.19 cells. A, Western blotting showing beclin-1, LC3, and p62 protein levels in hFOB 1.19 cells treated with AGE-BSA (150 μg/ml) for 0, 0.5, 1, 3, 6, 12, 24, or 48 h. B, Western blotting showing beclin-1, LC3, and p62 protein levels in hFOB 1.19 cells treated with AGE-BSA at various concentrations for 24 h. C, Western blotting showing beclin-1, LC3, and p62 protein levels in hFOB 1.19 cells treated with or without 3-MA (5 mm) incubated with AGE-BSA (150 μg/ml) or BSA (150 μg/ml) for 24 h. D–F, quantitative analysis of LC3, beclin-1, and p62 protein expression. Each bar represents mean ± S.E. of at least thee independent experiments. *, p < 0.05 versus control; **, p < 0.01 versus control; ##, p < 0.01 versus AGE-BSA treatment.