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. 2015 Oct 15;290(47):28189–28199. doi: 10.1074/jbc.M115.669499

FIGURE 5.

FIGURE 5.

RAGE/Raf/MEK/ERK pathway is involved in AGE-BSA induced autophagy. A, Western blotting showing RAGE protein levels in hFOB 1.19 cells treated with AGE-BSA at various concentrations for 24 h. B, quantitative analysis of RAGE protein expression. Each bar represents mean ± S.E. of at least three independent experiments. **, p < 0.01 versus control. C, Western blotting showing RAGE protein levels in hFOB 1.19 cells infected lentiviral with control shRNA or RAGE shRNA for 24 h. D, quantitative analysis of RAGE protein expression. Each bar represents mean ± S.E. of at least three independent experiments. **, p < 0.01 versus control; ##, p < 0.01 versus control shRNA treatment. E, Western blotting showing Raf/MEK/ERK pathway relative protein (c-Raf, MEK1/2, or ERK1/2) levels and autophagy relative protein (Beclin-1, LC3, or p62) levels in hFOB 1.19 cells treated with or without PD98059 (50 μm), control shRNA, or RAGE shRNA incubated with or without AGE-BSA (150 μg/ml) for 24 h. F and G, quantitative analysis of c-Raf, MEK1/2, ERK1/2, LC3, beclin-1, or p62 protein expression. Each bar represents mean ± S.E. of at least three independent experiments. **, p < 0.01 versus control; ##, p < 0.01 versus AGE-BSA treatment.