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. 2015 Oct 1;290(47):28299–28310. doi: 10.1074/jbc.M115.685511

TABLE 1.

PCR primer sets used for ChIP-PCR

PCR primer sequences were aligned with human genome (hg19). Sequences of PCR primer sets of γ-ALR jxn, Xp mono-HOR jxn, HOR satellite (D′), Xq mono-HOR jxn, and Xq sat jxn were from Mravinac et al. (32). NA, not applicable.

Genomic site Description Position (hg19)
A: γ-ALR jxn Forward: 5′-agcccgaggaaaatactggtgagg-3′ chX: 58319092–58319315
Reverse: 5′-gctgtctttctagtttttgtcgtgggttat-3′
B: Xp mono sat Forward: 5′-tgcagagggatatttgtaagcat-3′ chX: 58514700–58514948
Reverse: 5′-tgcttctgtctaattttcgtgtg-3′
C: Xp mono-HOR jxn Forward: 5′-aacgctgcgctatcaaagggaaagt-3′ chX: 58560457–58560769
Reverse: 5′-ggacatgtggagcgctttgtgc-3′
D: HOR satellite Forward: 5′-aaagggtgtttcgaacctga-3′ chX: 61718416–61718622
Reverse: 5′-tgaacatgccttttgatgga-3′
D′: HOR satellite Forward: 5′-ataatttcccataactaaacaca-3′ chX: 58605896–58606430
Reverse: 5′-tgtgaagataaaggaaaaggctt-3′
E: Xq mono-HOR jxn Forward: 5′-gacctcaaagcactctaaatacac-3′ chX: 61725908–61726403
Reverse: 5′-cttcacataaaaactagacagacag-3′
F: Xq mono sat Forward: 5′-aaaattgaggtttcaaaactgct-3′ chX: 61745729–61745968
Reverse: 5′-ttccttttcatagcgcactt-3′
G: Xq sat jxn Forward: 5′-cctgctgaatcaaaacaatggt-3′ chX: 62045588–62045976
Reverse: 5′-caaagaaggctgggtgagaag-3′
Universal α satellite Forward: 5′-cattctcagaaacttctttgtg-3′ NA
Reverse: 5′-cttctgtctagtttttatgtga-3′