TABLE 1.
Summary of the effects on Runx2/p57 mRNA expression and enrichment of the H3K4me3, H3K27ac, H3K27me3, and H4R3me2s marks at the Runx2-II/p57 P1 promoter after knockdown of the different chromatin modifying enzymes assessed in this study
ND, not determined during the study.
| Knockdown | Parameter Analyzed | Effect during osteoblast differentiation | Effect during myoblast differentiation |
|---|---|---|---|
| shWDR5 | Runx2 mRNA expression | Inhibited | ND |
| H3K4me3 enrichment | Decreases | ND | |
| shNO66 | Runx2 mRNA expression | Enhanced | Unaffected (remains repressed) |
| H3K4me3 enrichment | Unaffected (remains elevated) | Increases | |
| H3K27ac enrichment | Unaffected (remains elevated) | Unaffected (remains low) | |
| shJARID1B | Runx2 mRNA expression | Enhanced | Enhanced |
| H3K4me3 enrichment | Increases | Increases | |
| H3K27ac enrichment | Unaffected (remains elevated) | Increases | |
| shP300 | Runx2 mRNA expression | Inhibited | ND |
| H3K27ac enrichment | Decreases | ND | |
| shEzh2 | Runx2 mRNA expression | Unaffected (remains elevated) | Unaffected (remains repressed) |
| H3K27me3 enrichment | ND | Decreases | |
| shUTX | Runx2 mRNA expression | Inhibited | ND |
| H3K27me3 enrichment | Increases | ND | |
| shPRMT5 | Runx2 mRNA expression | Unaffected (remains elevated) | Unaffected (remains repressed) |
| H4R3me2s enrichment | ND | Decreases |