FIGURE 1.
Probing the aggregation of HEWL ultracentrifuged/ultrafiltrated fractions. A, samples were analyzed in bright field (left) and using crossed polarizers at 90° (right) with a Nikon Eclipse E400 POL microscope at ×4 magnification. B, UV-visible absorbance spectra for Congo Red alone and HEWL-separated fractions. All spectra were obtained from solutions containing 5 mm KH2PO4, 150 mm NaCl, pH 7.4. C, ThT absorbance readings of HEWL grown for 21 days at 65 °C in pH 2 conditions. HEWL control made fresh on the day and subject to no incubation can be also be seen. D, fluorescence emission spectra of HEWL samples upon binding the hydrophobic dye ANS. A and B are representative of 3 separate experiments. C and D, n = 1.