FIGURE 4.

Death pathway of PC12^undiff/diff cells treated with HEWL oligomers, sonicated fibrils, and mature fibrils was assessed using duel staining for annexin V-FITC/PI and flow cytometry. Cells were treated with a fraction for 24 h and then stained for the presence of both fluorescent markers. The x axis indicates the numbers of annexin V-FITC-stained cells. The y axis indicates the numbers of PI-strained cells. Nontreated PC12^undiff cells (A) and PC12^diff cells (E) are shown. PC12^undiff cells treated with 25 μm oligomer (B)-sonicated fibrils (C), and fibrils (D) show increased staining for both annexin V and PI. PC12^diff cells were treated with identical concentrations of oligomers (F), sonicated fibrils (G), and mature fibrils (H). All samples were gated according to untreated cells, and compensated using untreated cells stained for both dyes. Data are representative of one of three similar experiments.