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PslG displays glycoside hydrolase activity that is not required for Psl biosynthesis and biofilm formation. A, reducing sugar assay of cell-associated Psl with PslG(31–442) and catalytic variants. Error bars, S.E. from triplicate experiments. B, crystal violet biofilm assay with P. aeruginosa PAO1 ΔpelF PBADpsl and pslGE165Q, pslGE276Q, and pslGE165Q/E276Q chromosomal variants. PAO1ΔpelF PBADpsl ΔpslD was utilized as a negative control. C, Psl dot blot for strains listed in B.
