FIGURE 3.

Effect of ionic strength on septin assembly. A, a series of binding curves for Cdc11-labeled septin subunits assembled under varying ionic strength using the FRET corrected method. B, using PCA and 1:1 mixture of Cdc11^AF555:Cdc11^AF647, apparent Hill coefficients and EC₅₀ can be determined as a function of ionic strength. The light gray line illustrates the location of the EC₅₀ value. C, bar graphs illustrate the values of binding constants corresponding to the color-matched curves in A.