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. 2015 Sep 28;290(47):28388–28401. doi: 10.1074/jbc.M115.683128

FIGURE 5.

FIGURE 5.

Effect of deletion of the terminal subunit C-terminal extension on septin octamer assembly by FRET. A, Coomassie gels and typhoon scans of labeled septin octamers with AF555 and AF647 using end subunits of Shs1 ΔCTE and Cdc11 ΔCTE. B, binding curves for Cdc11AF555-Cdc11AF647 (red), Cdc11AF555-Cdc11 ΔCTEAF647 (cyan), and Cdc11 ΔCTEAF555-Cdc11 ΔCTEAF647 (magenta). C, binding curves for Shs1AF555-Cdc11AF647 (red), Shs1AF555-Cdc11 ΔCTEAF647 (cyan), Shs1 ΔCTEAF555-Cdc11AF647 (magenta), and Shs1 ΔCTEAF555-Cdc11 ΔCTEAF647 (black). For B and C, dots represent measured data, and color-coded lines represent the corresponding binding curve fit.