TABLE 3.
Parameters for the analysis of septin assembly with ΔCTE septins
Each value in the table represents the average of measurements made in triplicate and the ± values show the confidence interval calculated for p = 0.05. The donor was held at 25 nm, and FRET was measured in the absence and presence of increasing concentrations of acceptor up to 100 nm.
| FRET pair (donor-acceptor) | Apparent Kd | Apparent ABmax (fraction donor in FRET) |
|---|---|---|
| nm | ||
| Cdc11AF555-Cdc11 ΔCTEAF647 | 24.11 ± 11.45 | 1.15 ± 0.17 |
| Cdc11 ΔCTEAF555-Cdc11 ΔCTEAF647 | 17.68 ± 17.65 | 0.88 ± 0.24 |
| Shs1AF555-Cdc11 ΔCTEAF647 | 22.24 ± 16.45 | 1.06 ± 0.23 |
| Shs1 ΔCTEAF555-Cdc11AF647 | 30.38 ± 12.83 | 1.17 ± 0.17 |
| Shs1 ΔCTEAF555-Cdc11 ΔCTEAF647 | 22.83 ± 7.95 | 1.13 ± 0.12 |