TABLE 4.
Parameters for the analysis of septin assembly with Δα0 septins
Each value in the table represents the average of measurements made in triplicate, and the ± values show the confidence interval calculated for p = 0.05. The donor was held at 25 nm, and FRET was measured in the absence and presence of increasing concentrations of acceptor up to 100 nm.
| FRET pair (donor-acceptor) | Apparent Kd | Apparent ABmax (fraction donor in FRET) |
|---|---|---|
| nm | ||
| Cdc11AF555-Cdc11 Δα0AF647 | 19.49 ± 8.23 | 1.00 ± 0.12 |
| Cdc11 Δα0AF555-Cdc11 Δα0AF647 | 33.21 ± 19.17 | 1.07 ± 0.22 |
| Shs1AF555-Cdc11 Δα0AF647 | 8.13 ± 4.47 | 0.75 ± 0.10 |
| Shs1 Δα0AF555-Cdc11AF647 | 40.58 ± 58.31 | 1.28 ± 0.61 |
| Shs1 Δα0AF555-Cdc11 Δα0AF647 | 9.89 ± 8.93 | 0.80 ± 0.18 |