FIGURE 5.

CBP and P300 inhibition and depletion assert differential effects on expression of PLOD family members. A, PLOD2 and B, PLOD1 and PLOD3 mRNA expression of fibroblasts pre-treated for 1 h with 5 μm or 10 μm P300 inhibitor C646 or DMSO control, and co-treated with TGFβ1 or vehicle for additional 24 h. C, P300 and CBP mRNA expression of fibroblasts transfected with esiRNA against P300, CBP, or control. D, PLOD2 and E, PLOD1 and PLOD3 mRNA expression of fibroblasts transfected with CBP, P300, or control esiRNA and subsequently treated with TGFβ1 or vehicle for 48 h. Data are represented as mean ± S.E. (n = 3); *, p < 0.05; **, p < 0.01; ***, p < 0.001 unpaired Student's t test. Horizontal bars indicate both sets are significant to their individual control.