. 2015 Sep 23;290(47):28575–28583. doi: 10.1074/jbc.M115.666008

TABLE 1.

Oligonucleotide primers used in this study

	Sequence
yvrI fw (NcoI)	`ccatggatgaagcatcccatcgtgaagc`
yvrI rv (BamHI)	`ggatccttacttttccccctttttttgcgc`
yvrHa fw (NcoI)	`ccatgggtggacggcc agtttgaacaa`
yvrHa rv (BamHI)	`ggatccttatgagttctcatctaccatgtacaaaataaac`
yvrI S88C fw	`ggcgggagatcggctttcttgcgaaacagg`
yvrI S88C rv	`cctgtttcgcaagaaagccgatctcccgcc`
yvrI A142C fw	`gccacgatgcaggagattgcatgttcattagg`
YvrI A142C rv	`cctaatgaacatgcaatctcctgcatcgtggc`
YvrI S25C fw	`gttcaaaaacgtaatggaatgccctaacgaaaaag`
YvrI S25C Rv	`ctttttcgttagggcattccattacgtttttgaac`
yvrI S59C fw	`gattcgcatcttttgtattgattttgataagcggg`
yvrI S59C rv	`cccgcttatcaaaatcaatacaaaagatgcgaatc`
yvrI L168C fw	`cagaaagcagttgccggcgcaaaaaaaggggg`
yvrI L168C rv	`ccccctttttttgcgccggcaactgctttctg`
yvrHa C24S fw	`gagcacctgattgcaagcttttcaccgatgatc`
yvrHa C24S Rv	`gatcatcggtgaaaagcttgcaatcaggtgctc`
yvrHa C61S fw	`ggctgatatgcttttaagtcaggatgtaccgggg`
yvrHa C61S Rv	`ccccggtacatcctgacttaaaagcatatcagcc`
yvrHa S37C fw	`gaaaaaaactcagcaatacgtgctatcaagaaagagaag`
yvrHa S37C Rv	`cttctctttcttgatagcacgtattgctgagtttttttc`
yvrHa T15C fw	`gaaacaaaaagacgagtgttatgacattgagcacct`
yvrHa T15C Rv	`caggtgctcaatgtcataacactcgtctttttgtttc`
yvrHa I50C fw	`gagcaagagctgaagtgcaaaatgtttgaaaaggctg`
yvrHa I50C rv	`cagccttttcaaacattttgcacttcagctcttgctc`
PoxdC fw biotin	`biotin-gacagaactgaaagatcatgc`
PoxdC 98rv	`atggtaccctgcggaatgtcattttgttttttc`
PoxdC 54rv	`catttaaatgtgatagcgggcggcggcgg`
PyvrI fw biotin	`biotin-gcaaagtattttggaagacactaggcgg`
PyvrI 98rv	`gcttcacgatgggatgcttcattatctcc`
PyvrI 54rv	`ctccttacattttgaccgtaaaagactttg`
T7A1 fw biotin	`biotin-cggaattcggatccagatcccg`
T7A1 rv	`cgagtagcttcttcgattcgattcgcc`
M13 fw biotin	`biotin-gtaaaacgacggccagtgaattcgagc`
lacUV5 rv	`atggatcctgtgtgaaattgttatccgc`
σ⁷⁰-R2 fw	`atccatggttcaatgctccgttgctgaatatcc`
σ⁷⁰-R2 rv	`atgaattccgatcttcagcactttgcgg`
σ⁷⁰-R4 fw	`atccatggccaaagagccaatctcgatggaaac`
σ⁷⁰-R4 rv	`atggatccatcgtccaggaagctacgcagc`