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. 2015 Sep 23;290(47):28575–28583. doi: 10.1074/jbc.M115.666008

TABLE 2.

Promoter DNA templates used in the assays

The bold letter A denotes the transcription start site. Other bold letters denote the first non-template strand T or C nucleotide.

Sequence
Bs PoxdC EC+11 5′-gacagaactgaaagatcatgcaaaaaaataatttttcaatcgaagttgacttttcactggtttttttcacttaacaaaacAgaagggaaaaCgaaaggcctttcaccttctctttctgctatcacatttaaatg taaggaggaaacatttcatgaaaaaacaaaatgacattccgcag-3′
Bs PoxdC EC+20 5′-gacagaactgaaagatcatgcaaaaaaataatttttcaatcgaagttgacttttcactggtttttttcacttaacaaaacAgaagggaaaacgaaaggccTttcaccttctctttctgctatcacatttaaatgt aaggaggaaacatttcatgaaaaaacaaaatgacattccgcag-3′
Bs PoxdC EC+39 5′-gacagaactgaaagatcatgcaaaaaaataatttttcaatcgaagttgacttttcactggtttttttcacttaacaaaacAgaagggaaaacgaaaggccacacaccaccaccacccgcTatcacatttaaatg-3′
Bs PyvrI EC+10 5′-gcaaagtattttggaagacactaggcggaaagtagtagtttacttttttttgctttccttcatttaatttagtAgggaggcggTttgatggactatcaaagtcttttacggtcaaaatgtaaggagataatgaagcatcccatcgtgaagc
Bs PyvrI EC+19 5′-gcaaagtattttggaagacactaggcggaaagtagtagtttacttttttttgctttccttcatttaatttagtAgggaggaggtttgatggaCtatcaaagtcttttacggtcaaaatgtaaggag-3′
Ec T7A1 EC+23 5′-cggaattcggatccagatcccgaaaatttatcaaaaagagtattgacttaaagtctaacctataggatacttacagccAtcgagagggacacgggcgaagcTtggcgaatcgaatcgaagaagctactcg-3′
Ec lacUV5 EC+15 5′-taggcaccccaggcttgacactttatgcttcggctcgtataatgtgtggAattgtgaggagaggCggataacaatttcacacaggatccat-3′
Ec lacUV5 EC+25 taggcaccccaggcttgacactttatgcttcggctcgtataatgtgtggAattgtgaggagaggtggataataaCggataacaatt tcacacagg
Ec lacUV5 EC+40 taggcaccccaggcttgacactttatgcttcggctcgtataatgtgtggAattgtgaggagaggtggataataatttaatataggattaCaggcggataac