. 2015 Nov 20;5:16598. doi: 10.1038/srep16598

Table 2. Q-RT-PCR verification for genes expression at different times with 0.0005 and 0.001 g/L ZnSO₄.7H₂O supplementation.

Gene	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	RNA-Seq	Q-RT-PCR (Zinc/Control)
Gene	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	RNA-Seq	8 h(0.001)	16 h(0.001)	32 h(0.001)	16 h(0.0005)
CAC0234	CAATGGCAGCAGGAATGGT	TGGTATTGCGCCTTCTGTTATG	2.76	3.27	4.03	0.78	4.16
CAC0386	CAAACTTCCAGAAAGCGTACCA	AACACCAAACCACCAGAAGAAAG	5.19	5.96	7.78	0.66	6.33
CAC0570	AAATGCTTGGAGACGGTTTTG	AGTCCGCCTTCTGTTGTTATTG	3.62	4.93	4.98	0.53	3.82
CAC1353	GCACTTCCAGCTGCATGTTTAG	TCCAAGAAGCATACCAGAAACCT	1.76	1.46	2.01	0.46	2.05
CAC1354	AAGTAGGGCAGCCGATTATAAAAGT	TGGTTTCCAGCGTCAACATC	1.77	1.94	2.27	0.36	2.33
CAC1342	GGAATGAAGCAATTACCTGGACTT	GCTGTTTTCCAATCGCCTTC	4.84	3.33	6.07	0.59	5.74
CAC2613	ATTTCTCTCAAAGAGGAAGGTTCAC	TTTGTGGATGTGGAAAGACAGG	1.21	1.55	1.89	0.72	1.47
CAC0078	GGTTCTGGCGTCTTTTATAATCGT	TTTAGCTTGTCCCTCAGTTTAGCTC	0.65	0.70	0.66	0.83	0.79
CAC2873	ACAGTTTCTCCCTTTCTGCCTTT	AAGATGAGTTTGCTCTTGCATCAC	1.46	1.42	2.45	0.67	1.97
CAC3298	CCCTACGCCGTGTGTTATGTC	TGAGGCAAGAGCCAATCTAATGT	1.35	1.91	2.57	0.65	2.28
CAP0164	TTCGATAGCTCAGTTTCGTTTTCAC	CCACCCATACCAGAGAGCATTT	1.62	1.78	2.93	0.75	2.06
CAC0905	TGGATTACAAGCAGCCATTTC	CTGTTCCCGTAAGCTCCTCTTT	1.00	Housekeeping gene

Table 2. Q-RT-PCR verification for genes expression at different times with 0.0005 and 0.001 g/L ZnSO4.7H2O supplementation.