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. 2015 Nov 20;5:16975. doi: 10.1038/srep16975

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(A) Cell binding assayed by FACS. After ELISA quantitation, the cell supernatants containing equal amount of the secreted interferons (SIFα and IFNα) were incubated with CFPAC1 cancer cells. The FITC conjugated-IFNα antibody was used for FACS quantitation of the ability of cell binding. The FITC mouse IgG was used as the isotype control. The Median Fluorescence Intensity (MFI) was calculated to evaluate the cell binding. (B) Relative MFI in SIFα- and IFNα-treated cells. MFI was standardized as the percentage of the isotype control group. Data represent mean ± SD of three independent repeats. * Significantly higher binding to the pancreatic cancer cells (p < 0.05).