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Presence of retinal pigment epithelium (RPE) layer in hESC-derived retinal tissue. Immunostaining of hESC-derived retinal tissue (6 weeks) with two mature RPE markers, EZRIN, an actin-binding protein, and ERM-binding phosphoprotein50/sodium–hydrogen exchanger regulatory factor1 (EBP50/NHERF1), shows strong staining in the apical side. Inset represents the magnification of the area marked with an asterisk. 4′, 6-diamidino-2-phenylindole (DAPI)-stained nucleus. Scale bar: 20 μm.
