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. 2015 Aug 18;24(23):2778–2795. doi: 10.1089/scd.2015.0144

FIG. 3.

FIG. 3.

OTX2 [+] cells in hESC-derived retinal tissue are postmitotic. (a) Immunolabeling of hESC-derived retinal tissue (6 weeks) with antibodies to OTX2 and postmitotic neuronal marker, MAP2. Insets are magnifications of framed area showing no colocalization between OTX2 and MAP2. Arrows point to the presence of OTX2 [+] cells in the ciliary margin-like zone. Scale bar: 50 and 10 μm. (b–e) Confocal images (single optical sections) showing no colocalization of OTX2 and Ki67 (marker of cell division) in hESC-derived retinal tissue, indicating that OTX2 [+] cells are postmitotic. DAPI-stained nucleus. Scale bar: 100 μm. (d′, e′) Confocal (z-stack) images of the area marked with an asterisk in (b–e). The confocal image was virtually resectioned at the x and y planes to confirm the identity of the double-labeled cell. Scale bar: 50 μm.