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. 2015 Nov 19;15:919. doi: 10.1186/s12885-015-1929-y

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© Koehler et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Autophagy regulation by Obatoclax in 3D long term cell culture. a HT29 cells were seeded on glass slides, grown for 7 days and treated with 0.25 μM obatoclax. Immunhistochemical staining was done for p62 and LC3b. b HT29 cells in scaffolds after 7 days treatment with obatoclax. Immunhistochemical staining for p62 and LC3b. Representative pictures for at least 3 experiments are shown. Scale bar indicates 200 μm for longitudinal sections and 50 μM for corresponding insets. DMSO served as vehicle