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. 2015 Nov 19;15:919. doi: 10.1186/s12885-015-1929-y

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© Koehler et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — LC3 accumulates in autophagosomes in cells treated with Obatoclax. a Immunofluorescence staining for LC3 in untreated (upper panel), Chloroquine treated (30 μM, middle panel) and obatoclax treated (0.25 μM, lower panel) cells. Representative pictures are shown. Assays were done at least three times. b Analysis of LC3 aggregates per cell. Values are expressed as mean ± SD. *** = p < 0.001