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. 2015 Nov 19;15:919. doi: 10.1186/s12885-015-1929-y

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© Koehler et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Obatoclax blocks autophagy independent of canonical autophagy signaling. a and b Representative western blotting for HT29 cells treated with obatoclax. siRNA mediated knockdown of Atg12 and Atg7 is shown in (a), siRNA mediated knockdown of Mcl-1 or Beclin-1 is shown in (b). c Flow cytometric analysis for apoptosis induction in full supplemented (left) or starving (right) HT29 cells after knockdown of Atg7 or Beclin-1 and treatment with obatoclax. Values are expressed as mean ± SD. Staurosporine 2 μM for 2 h served as a positive control for cell death induction