Fig. 5.

Zymosan injection facilitated the trafficking of AMPA receptor into membrane and phosphorylation of GluA1 at the Ser845 site in the ACC. a Representative Western blot for GluA1 and GluA2/3 on membrane protein in the ACC obtained from control and zymosan-injected mice. b-c The expressions of GluA1 and GluA2/3 on membrane in the ACC were significantly enhanced on day 7, and 14 after mice injected with zymosan. d Representative Western blot for GluA1 and GluA2/3 on cytosolic protein in the ACC obtained from control and zymosan-injected mice. e-f) The expressions of GluA1 and GluA2/3 on cytosol in the ACC had no difference among three groups. g Representative Western blot for phosphorylated GluA1 at the Ser845 site in the ACC obtained from control and zymosan-injected mice. h The phosphorylation of GluA1 at the Ser845 site was significantly enhanced in the ACC on day 1, 7, and 14 after mice injected with zymosan. N = 6 mice per group, * P < 0.05, ** P < 0.01 vs. control