Fig. 2.

Phylogenetic tree highlighting the position of Tolumonas lignolytica BRL6-1^T among the Aeromonadales. a The phylogenetic tree based on 16S ribosomal RNA gene sequence was inferred using the Neighbor-Joining method [54] within MEGA6 [55]. Bootstrap values of 1000 replicate trees are shown at the branches [56]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Jukes-Cantor method [57] and are in units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated, creating a total of 1234 positions in the final dataset. GenBank accession numbers are shown in parentheses after strain numbers. Type strains are indicated with a superscript T. Organisms with genomes available are indicated by an asterisk before the name. b Whole genomes were hierarchically clustered based on COG profiles using tools in IMG [58]. T. lignolytica BRL6-1^T is indicated in bold in both phylogenetic trees