Table 8.
Enzymes involved in lignin degradation with homologues in BRL6-1T genome
| Enzyme | Gene name | Characterized in | # of copies in genome | E value | Gene annotation in BRL6-1T genome |
|---|---|---|---|---|---|
| Cα-dehydrogenase involved in b-aryl ether cleavage | ligD | Sphingomonas paucimobilis SYK-6 | 2 | 6e-12; 4e-10 | 1)3-oxoacyl-[acyl-carrier-protein] reductase (EC 1.1.1.100) 2)hypothetical protein |
| Beta-etherase | ligF | Sphingomonas paucimobilis SYK-6 | 1 | 4e-11 | Glutathione S-transferase |
| Feruloyl-CoA synthetase | fcs | Pseudomonas putida strain KT2440 | 1 | 1e-10 | Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II |
| Feruloyl-CoA hydratase/lyase | ferB | Sphingomonas paucimobilis | 1 | 7e-15 | 1,4-Dihydroxy-2-naphthoyl-CoA synthase (EC 4.1.3.36) |
| Feruloyl-CoA hydratase/lyase | ferB2 | Sphingomonas paucimobilis | 1 | 3e-32 | 1,4-Dihydroxy-2-naphthoyl-CoA synthase (EC 4.1.3.36) |
| Vanillin dehydrogenase | ligV | Sphingomonas paucimobilis | 5 | 4e-66; 3e-65; 2e-62; 2e-35; 8e-08 | 1) succinate semialdehyde dehydrogenase (EC 1.2.1.16) 2) gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase 3) NAD-dependent aldehyde dehydrogenases 4) succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71) 5) acetaldehyde dehydrogenase (EC 1.2.1.10)/alcohol dehydrogenase AdhE (EC 1.1.1.1) |
| Vanillate O-demethylase oxidoreductase | vanB | Pseudomonas syringae pv. tomato strain DC3000 | 3 | 9e-18; 7e-14; 9e-12 | 1)Hemoglobin-like flavoprotein 2)Flavodoxin reductases (ferredoxin-NADPH reductases) family 1 3)Predicted ferric reductase |
| 3-carboxymuconate cycloisomerase | pcaB | Pseudomonas putida | 2 | 9e-13; 5e-09 | 1)Adenylosuccinate lyase (EC 4.3.2.2) 2)argininosuccinate lyase (EC 4.3.2.1) |
| 4-carboxymuconolactone decarboxylase | pcaC | Pediococcus acidilactici D3 | 3 | 6e-30; 7e-27; 1e-06 | Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit (all 3) |
| Β-ketoadipate enol-lactone hydrolase | pcaD | Acinetobacter baumannii strain SDF | 2 | 5e-15; 5e-13 | 1)carboxylesterase BioH (pimeloyl-CoA synthesis) (EC 3.1.1.85) 2)Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) |
Enzyme protein sequences were obtained from UniProt. E values are based on blastp results performed in img/er against the T. lignolytica BRL6-1T genome