Table 3.

Gradation of colposcopist judgment attributed to biopsy sites corresponding to specimen A and B before and after revision analysis in senior and junior group

Examiner groupa	Colposcopist evaluationb	senior N (%)	junior N (%)	p value
Routine histological Analysis	A < B	10 (26.3)	16 (51.6)	.03
	A > B	28 (73.7)	15 (48.4)
Revised histological Analysisc	A < B	12 (27.3)	19 (50.0)	.03
	A > B	32 (72.7)	19 (50.0)

Statistical analysis was performed considering only patients with definitive diagnosis of CIN. A colposcopist evaluation reporting A = B was considered not informative and excluded from the statistical analysis

^aIn senior group colposcopic examination and biopsies were performed by experienced colposcopists; in junior group post-graduate doctors with one-year experience in Unit of Cervicovaginal Pathology performed the diagnostic procedures

^bAccording to the judgment of the colposcopist biopsy A was considered the most suspicious and representative of the whole cervical lesion and biopsy B was considered additional but not required to obtain histological diagnosis

^cRevision analysis: analysis performed by experienced pathologist. In uncertain cases immunohistochemical stains were used, particularly, antibody against ki67 to evaluate the proliferative activity and p16 protein expression to determine the different degrees of CIN