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. 2015 Sep 22;24(24):6899–6909. doi: 10.1093/hmg/ddv390

Figure 4.

Figure 4.

Spt1C129W expression in class IV da neurons reduces membrane trafficking to the plasma membrane and induces membrane trafficking defects. (A) Class IV da neurons expressing the membrane marker mCD8-EGFP and Spt1C129W develop a perinuclear accumulation of EGFP not observed in wild-type animals. (B) FRAP in class IV da neuron secondary dendrites expressing the membrane marker mCD8-EGFP, driven by the ppk-GAL4 construct is slower when co-expressing Spt1C129W. An area of 45 × 15 pixels was bleached using 100% laser power at 488 nm for 200 iterations and monitored for 200 s. t-half for control dendrites is 20 s (n = 25) and 31 s for cells expressing SPT1C129W (n = 26) with a significant difference of P ≤ 0.01. Student's t-test, t = 2.03, 33 df. (C) Examples of fluorescence recovery in wild-type neurons or neurons expressing Spt1C129W. Scale bar = 10 µm. (D) Spt1C129W expressing neurons show an accumulation of an ER marker (KDEL-EGFP) in the cell body compared with wild-type. Scale bar = 10 µm.