Figure 5.
Areas of RPE thinning and subretinal ectopic pigmented cells are observed in Adamtsl4tvrm267 mice. (A) H&E staining of methanol/acetic acid fixed sections shows RPE of uniform thickness in a 1-year-old WT (B6) mouse. (B) Age-matched mutant mice exhibit variable RPE thickness as well as perturbations to the outer retina. (C) Closer view of the H&E stained section (yellow box) showing thinning of RPE (arrow) and an ectopic binucleated (white arrowheads) pigmented cell adjacent to the thinned area. (D) Electron micrograph of subretinal ectopic pigments observed in Adamtsl4tvrm267 mice. Dashed lines highlight the cells rich in electron dense granules. Asterisk indicates amorphous material. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; RPE, retinal pigmented epithelium; Ch, Choroid; OS, outer segment; N, nucleus. Scale bars: (A–C) 25 µm; (D) 2 µm.