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Rescue of HPRT− cells with mHP-I-RT
| Transfected plasmid | Encoded minigene | Experiment 1a | Experiment 2 | Experiment 3 |
|---|---|---|---|---|
| pBT/PGK-HPRT (R1) | mHPRT (wt) | 189 | 241 | 266 |
| pBT/PGK-HP-I-RT (R1) | mHP-I-RT | 198 | 213 | 259 |
| pBT/PGK-HP-I*-RT (R1) | mHP-I*-RT | 0 | 0 | 0 |
aIn each experiment, plasmids expressing the indicated minigene were individually electroporated into 1 million HPRT− cells which were then selected in HAT medium to generate the indicated numbers of HATR colonies.
