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Frequencies of gc/HR induced by different HPRT-targeted nucleasesa
| Nuclease | I-SceI | I-SceI | gRNA1/Cas9 | gRNA3/Cas9 | gRNA3/Cas9 | gRNA4/Cas9 |
|---|---|---|---|---|---|---|
| Template | − | + | + | − | + | + |
| Experiment 1 | UD | 0.093 | UD | UD | 0.013 | nt |
| Experiment 2 | nt | 0.12 | nt | nt | 0.016 | 0.019 |
UD, undetectable; nt, not tested.
aFigures show % of colony forming units that became HATR after lipofection of clone 3B with the indicated nucleases with or without repair template (pJB2).
