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. 2015 Oct 1;24(24):7111–7120. doi: 10.1093/hmg/ddv410

Figure 3.

Figure 3.

SCN4A expression in the mouse and human brain. (A) Mouse brain expression. Column 1: 100 bp ladder; Column 2: multiplex RT-PCR products of SCN2A and ACTB from brain RNA; Column 3: multiplex RT-PCR products of SCN2A and ACTB from muscle RNA; Column 4: multiplex RT-PCR products of SCN4A and ACTB from brain RNA; Column 5: multiplex RT-PCR products of SCN4A and ACTB from muscle RNA (Column 5). Note that SCN2A and SCN4A are expressed in both skeletal muscle and brain. The ubiquitously expressed ACTB encoding β-actin served as a control for the reactivity of the mix only. (B) Human brain expression. The expressions of SCN4A and other SCN genes known to be expressed in the human brain were evaluated by absolute quantification using the standard curve method. The y-axis represents the mRNA expression (in number of copies), and the x-axis represents the SCN genes tested in the cerebral cortex of the human brain.