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. 2015 Oct 3;24(24):7132–7150. doi: 10.1093/hmg/ddv413

Figure 1.

Figure 1.

Increased levels of Aβ-related peptides in ANPC brains without any alteration in APP holoprotein or soluble APP fragments. (A and B) Real-time qPCR and immunoblotting analysis depicting APP mRNA (A) and holoprotein (∼98 kDa, B) levels, respectively, in the cerebellum of WT, APP-Tg, Dhet and ANPC mice. (C and D) Immunoblots and corresponding histograms showing increased α- and β-CTFs (∼10–12 kDa, C) but unaltered sAPPα and sAPPβ (D) fragments in the cerebellum of APP-Tg, Dhet and ANPC mice. (E and F) Age-dependent increase in human Aβ1–40 (E) and Aβ1–42 (F) levels in APP-Tg, Dhet and ANPC cerebellar homogenate, detected by ELISA. All blots were re-probed with anti-β-actin to monitor protein loading. Values are means ± SEM from 4 to 10 (i.e. ELISA, n = 6–10, qPCR and western blot, n = 4) mice/genotype/age. *P < 0.05; **P < 0.01; ***P < 0.001.