Figure 9.

EL cholesterol sequestration increases oxidative stress and reduces cell viability. (A–C) Representative immunoblots of protein carbonyl levels, indicative of oxidative stress, in the cerebellum of 4- and 10-week-old WT, APP-Tg, Dhet and ANPC mice (A and B; n = 4 mice/genotype/age) and N2a-APP/ANPC cells (C) with and without exposure to 500 μm H₂O₂ for 3 h. (D) Histogram depicting MTT viability of N2a-APP and N2a-ANPC cells following 3 h treatment with H₂O₂ (0–500 μm). (E–G) Histograms showing reduced H₂O₂-induced toxicity of the cells upon 24 h pre-treatment with the β-secretase inhibitor BIV (10 μm, E), the γ-secretase inhibitor DAPT (1 μm, F) and the autophagy inducer rapamycin (10 μm, G). All blots were re-probed with anti-β-actin. Culture data represent means ± SEM from four independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.