Table 1.
Primary antibodies used
| Antibody | Type | IF dilution | WB dilution | Source |
|---|---|---|---|---|
| APP (clone Y188) | Monoclonal | 1:250 | 1:5000 | Abcam Inc. |
| APP (clone C1/6.1) | Monoclonal | 1:1000 | n/a | Covance Corp. |
| APP (clone 22C11) | Monoclonal | 1:2000 | n/a | EMD Millipore Co. |
| APP (clone CTM1) | Polyclonal | 1:2000 | n/a | Kind gift from Dr G. Thinakaran, University of Chicago, USA |
| Cathepsin D (CatD) | Polyclonal | 1:200 | n/a | Santa Cruz Biotechnology, Inc. |
| Lamp1 (clone ID4B) | Monoclonal | 1:500 | n/a | Developmental studies, Hybridoma Bank, Univ. of Iowa, USA |
| LC3 | Polyclonal | 1:1000 | 1:1000 | MBL International Corp. |
| Nicastrin | Polyclonal | n/a | 1:500 | Santa Cruz Biotechnology, Inc. |
| p62 (sequestosome-1) | Monoclonal | 1:100 | 1:1000 | EMD Millipore Co. |
| Presenilin1-NTF (PS1-NTF) | Polyclonal | n/a | 1:3000 | Kind gift from Dr G. Thinakaran, University of Chicago, USA |
| Proteasome subunit beta type 2 (β2) | Monoclonal | n/a | 1:1000 | Abcam Inc. |
| Rab5 | Polyclonal | 1:200 | n/a | Abcam Inc. |
| Rab7 | Polyclonal | 1:500 | n/a | Sigma-Aldrich, Inc. |
| sAPPα (2B3) | Monoclonal | n/a | 1:1000 | IBL Co., Ltd |
| sAPPβ-sw (6A1) | Monoclonal | n/a | 1:3000 | IBL Co., Ltd |
| Ubiquitin | Polyclonal | 1:250 | 1:200 | Santa Cruz Biotechnology, Inc. |
| β-Actin | Monoclonal | n/a | 1:5000 | Sigma-Aldrich, Inc. |
| β-Amyloid, 1–16 (6E10) | Monoclonal | 1:1000 | 1:3000 | Covance Corp. |
| β-Amyloid, 17–24 (4G8) | Monoclonal | 1:1000 | n/a | Covance Corp. |
IF, immunofluorescence; WB, western blotting; n/a, not used in that application.