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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cancer Lett. 2015 Aug 28;369(1):175–183. doi: 10.1016/j.canlet.2015.08.012

Figure 1. RGS10 suppression induces the phosphorylation of 4E-BP1 in ovarian cancer cells under low nutrient conditions.

Figure 1

(A) OVCAR-3, HeyA8 and SKOV-3 cells were transfected with the siGENOME RISC-Free control siRNA (siRISC) or siRGS10 and kept in serum-free medium prior to immunoblotting detection for phospho-4E-BP1 at Thr37/46 and actin. (B) Repeated experiments (n=3–5) of phospho-4E-BP1 in HeyA8 and SKOV-3 cells were quantified using ImageJ. A significant increase in the phosphorylation among siRGS10 cells (*p<0.05 vs siRISC) is observed. (C) HeyA8, OVCAR-3 or (D) SKOV-3 cells were processed for detection of RGS10 protein expression and actin.