FIGURE 1.

Induction of Trim cluster genes in macrophages and DCs. (A) Schematic diagram of the Trim cluster genes and the Trim5 group region in the mouse genome. The Trim cluster encompasses ∼17 Mb in chromosome 7 (gray bar). The Trim5 region is expanded in the mouse genome to form the Trim5 group, which contains 11 Trim genes within the ∼250-kb space (blue bars with each gene in red). In this study, Trim12c has been analyzed in detail. Trim12a encodes a truncated protein identical to TRIM12c but lacking the SPRY domain. The predicted amino acid sequence of TRIM5 is virtually identical to that of TRIM12c. (B) RAW 264.7 cells stimulated with IFN-γ (100 U/ml) for 24 h, NDV at 640 HA units, or MCMV at a multiplicity of infection of 5 for 24 h or IFN-γ (overnight) plus CpG (6 h, 1 μg/ml), and mRNA levels of indicated Trim genes were measured by qRT-PCR. (C) BM-derived macrophages were stimulated by IFN-β or IFN-γ for indicated times, and mRNA levels of indicated Trim genes were tested as above. (D) Flt3L-derived BMDCs were stimulated with CpG (1 μg/ml) for indicated times, and Trim gene expression was detected as above. mRNA levels of Trim genes were normalized by the hypoxanthine–guanine phosphoribosyltransferase mRNA. Values in this figure represent the average of three independent experiments ± SD.