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. 2015 Oct 30;195(11):5358–5366. doi: 10.4049/jimmunol.1500339

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Copyright © 2015 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

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FIGURE 4. — Interaction between WDR82 and TRAF3. (A) Overexpressed WDR82 interacts with TRAF3. HEK293 cells were cotransfected with Myc-tagged WDR82 plasmids and HA-tagged RIG-I, VISA, MITA, TRAF3, TBK1, IKKε, and IRF3. Lysates were coimmunoprecipitated with anti-HA and Western blotted with anti-HA and anti-Myc. (B) Endogenous interaction between TRA3 and WDR82. HEK293 cells were infected with SeV, and coimmunoprecipitation and Western blotting were performed with the indicated Ab. (C) Sketch map of TRAF3 domains. (D) Mapping of TRAF3–WDR82 interacting domain. HA-tagged TRAF3 truncations and Flag-tagged WDR82 were cotransfected into HEK293 cells. Coimmunoprecipitation and immunoblot assays were performed as indicated. (E and F) WDR82 is colocalized with TRAF3 and IKKε. C-terminal EGFP-tagged WDR82 and HA-tagged TRAF3 or IKKε were cotransfected into U2OS cells. Immunofluorescence staining was performed as indicated. (E and F) Original magnification ×600.