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. 2015 Apr 14;15(8):2170–2179. doi: 10.1111/ajt.13241

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© 2015 The Authors. American Journal of Transplantation Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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TCR analysis in stem cell donors before and after G-CSF induced mobilization. (A) Mean Vβ subgroup usage of TCRs from CD4⁺ or CD8⁺ T cells of 23 donors before (pre) and after (post) G-CSF induced stem cell mobilization. The seven most frequent Vβ subgroups were illustrated individually; the remaining subgroups were grouped together. The nomenclature according to the international ImMunoGeneTics information system (IMGT) was used. (B) Mean percentage of overlaps of individual clonotypes in CD4⁺ or CD8⁺ T cells of the 23 donors pre– and post–G-CSF–induced stem cell mobilization. (C and D) Diversity dot plots of CD4⁺ and CD8⁺ T cells pre– and post–G-CSF mobilization. Each dot represents the number of unique clonotypes detected in individual donors. (C) The number of clonotypes in CD4⁺ T cells differs significantly before and after G-CSF mobilization from the number of clonotypes in the CD8⁺ compartment (****p < 0.0001; n = 44). Bars show mean values. (D) The number of clonotypes in CD4⁺ and CD8⁺ T cells is not affected by G-CSF administration. (E) Scatter plots of CD4⁺ and CD8⁺ clonotype numbers and the age for stem cell donors pre– and post–G-CSF mobilization. The number of individual CD8⁺ clonotypes decreases with increasing donor age irrespective of G-CSF mobilization (preMOB: r = −0.42; p = 0.052; postMOB: r = −0.42; p = 0.051; n = 22). For CD4⁺ T cells, no negative correlation was found. CD4⁺ T cells preMOB; CD4⁺ T cells postMOB; CD8⁺ T cells preMOB; CD8⁺ T cells postMOB.

Inline graphic — TCR analysis in stem cell donors before and after G-CSF induced mobilization. (A) Mean Vβ subgroup usage of TCRs from CD4⁺ or CD8⁺ T cells of 23 donors before (pre) and after (post) G-CSF induced stem cell mobilization. The seven most frequent Vβ subgroups were illustrated individually; the remaining subgroups were grouped together. The nomenclature according to the international ImMunoGeneTics information system (IMGT) was used. (B) Mean percentage of overlaps of individual clonotypes in CD4⁺ or CD8⁺ T cells of the 23 donors pre– and post–G-CSF–induced stem cell mobilization. (C and D) Diversity dot plots of CD4⁺ and CD8⁺ T cells pre– and post–G-CSF mobilization. Each dot represents the number of unique clonotypes detected in individual donors. (C) The number of clonotypes in CD4⁺ T cells differs significantly before and after G-CSF mobilization from the number of clonotypes in the CD8⁺ compartment (****p < 0.0001; n = 44). Bars show mean values. (D) The number of clonotypes in CD4⁺ and CD8⁺ T cells is not affected by G-CSF administration. (E) Scatter plots of CD4⁺ and CD8⁺ clonotype numbers and the age for stem cell donors pre– and post–G-CSF mobilization. The number of individual CD8⁺ clonotypes decreases with increasing donor age irrespective of G-CSF mobilization (preMOB: r = −0.42; p = 0.052; postMOB: r = −0.42; p = 0.051; n = 22). For CD4⁺ T cells, no negative correlation was found. CD4⁺ T cells preMOB; CD4⁺ T cells postMOB; CD8⁺ T cells preMOB; CD8⁺ T cells postMOB.