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. 2015 Apr 14;15(8):2170–2179. doi: 10.1111/ajt.13241

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© 2015 The Authors. American Journal of Transplantation Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Donor TCRβ diversity and clinical correlation. (A) Diversity dot plots of CD4⁺ and CD8⁺ T cells of cytomegalovirus (CMV)-seropositive or -seronegative donors before (pre) and after (post) G-CSF mobilization. Only for the CD4⁺ T cell compartment postmobilization a significant difference could be displayed between CMV-seropositive and -seronegative donors (*p < 0.05; neg. n = 14; pos. n = 8). (B) Diversity dot plots of CD4⁺ and CD8⁺ TCRβ rearrangements of G-CSF–mobilized donors premobilization and postmobilization according to patients either reactivated (“Yes”) or not (“No”) with CMV (“Yes”: CD4⁺ n = 10; CD8⁺ n = 11) or Epstein–Barr virus (EBV; “Yes”: CD4⁺ n = 7; CD8⁺ n = 7). Cases with CMV and/or EBV reactivation presented significantly lower numbers of unique clonotypes in CD4⁺ T cells post–G-CSF mobilization (*p < 0.05). In CD8⁺ T cells, no significant difference could be detected for virus reactivation. (C) Diversity dot plots of CD4⁺ and CD8⁺ T cells of donor T cells premobilization and postmobilization according to patients who either exhibited acute graft-versus-host disease (aGvHD; “Yes”) or not (“No”). No significant correlation could be detected between donor T cell diversity and aGvHD appearance in patients. CD4⁺ T cells preMOB; CD4⁺ T cells postMOB; CD8⁺ T cells preMOB; CD8⁺ T cells postMOB.

Inline graphic — Donor TCRβ diversity and clinical correlation. (A) Diversity dot plots of CD4⁺ and CD8⁺ T cells of cytomegalovirus (CMV)-seropositive or -seronegative donors before (pre) and after (post) G-CSF mobilization. Only for the CD4⁺ T cell compartment postmobilization a significant difference could be displayed between CMV-seropositive and -seronegative donors (*p < 0.05; neg. n = 14; pos. n = 8). (B) Diversity dot plots of CD4⁺ and CD8⁺ TCRβ rearrangements of G-CSF–mobilized donors premobilization and postmobilization according to patients either reactivated (“Yes”) or not (“No”) with CMV (“Yes”: CD4⁺ n = 10; CD8⁺ n = 11) or Epstein–Barr virus (EBV; “Yes”: CD4⁺ n = 7; CD8⁺ n = 7). Cases with CMV and/or EBV reactivation presented significantly lower numbers of unique clonotypes in CD4⁺ T cells post–G-CSF mobilization (*p < 0.05). In CD8⁺ T cells, no significant difference could be detected for virus reactivation. (C) Diversity dot plots of CD4⁺ and CD8⁺ T cells of donor T cells premobilization and postmobilization according to patients who either exhibited acute graft-versus-host disease (aGvHD; “Yes”) or not (“No”). No significant correlation could be detected between donor T cell diversity and aGvHD appearance in patients. CD4⁺ T cells preMOB; CD4⁺ T cells postMOB; CD8⁺ T cells preMOB; CD8⁺ T cells postMOB.