Table 3.
TCRβ repertoire diversity and clinical correlation
| CD4+ T cells |
CD8+ T cells |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Donor TCRβ repertoire | >Mean | <Mean | Total | p-value χ2-test | >Mean | <Mean | Total | p-value χ2-test | |
| Donor type | MUD | 9 | 5 | 14 | 8 | 7 | 15 | ||
| SIB | 5 | 3 | 8 | 0.933 | 2 | 5 | 7 | 0.277 | |
| Donor age | <40 | 5 | 2 | 7 | 5 | 2 | 7 | ||
| >40 | 8 | 5 | 13 | 0.658 | 3 | 10 | 14 | 0.035 | |
| Patient | |||||||||
| Survival | Alive | 9 | 7 | 16 | 8 | 9 | 17 | ||
| Dead | 5 | 1 | 6 | 0.240 | 2 | 3 | 5 | 0.781 | |
| TRM | Alive | 11 | 7 | 18 | 9 | 10 | 19 | ||
| Dead | 3 | 1 | 4 | 0.601 | 1 | 2 | 3 | 0.650 | |
| Relapse | No | 10 | 6 | 16 | 6 | 10 | 16 | ||
| Yes | 4 | 2 | 6 | 0.856 | 4 | 2 | 6 | 0.221 | |
| EBV reactivation | No | 12 | 3 | 15 | 7 | 8 | 15 | ||
| Yes | 2 | 5 | 7 | 0.020 | 3 | 4 | 7 | 0.867 | |
| CMV reactivation | No | 10 | 2 | 12 | 5 | 6 | 11 | ||
| Yes | 4 | 6 | 10 | 0.035 | 5 | 6 | 11 | 1.000 | |
| aGvHD | No | 4 | 2 | 6 | 2 | 4 | 6 | ||
| Yes | 10 | 6 | 16 | 0.856 | 8 | 8 | 16 | 0.484 | |
Contingency table of donor CD4+ and CD8+ T cell diversity post–G-CSF mobilization in correlation with clinical parameters of the recipients. Groups were divided according to their clonotype numbers above or below the compartment mean (CD4+ mean = 2490 clonotypes; CD8+ mean = 1661 clonotypes). According to this classification, a correlation between lower donor CD4+ TCRβ diversity and virus reactivation (CMV and EBV) was significant. In addition, a significant correlation could be demonstrated between lower CD8+ TCRβ diversity and the age of the donor.
aGvHD, acute graft-versus-host disease; χ2-test, chi-squared test; TRM, transplant-related mortality.