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. 2015 Feb 5;87(3):244–253. doi: 10.1002/cyto.a.22614

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© 2015 The Authors. Published by Wiley Periodicals, Inc. on behalf of ISAC.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Youden-diagram of CD4+ cell concentrations for dilutions 1 and 2. Youden-diagram of the results obtained for both dilutions. The two outliers are indicated by open circles. The mean values of participants= 300.4 μL⁻¹ and= 302.9 μL⁻¹ are plotted as vertical and horizontal lines, respectively. The diagonal with unity slope was inserted to illustrate that the results obtained from both dilutions are consistent with a linear relation within the uncertainties reached. The middle dashed square indicates boundaries corresponding to ±15% deviation from the respective mean values. If the outliers are disregarded, 2 out of 21 values are outside of this range, that is, 90% of the participants' results are within a limit of ±15%.

Inline graphic — Youden-diagram of CD4+ cell concentrations for dilutions 1 and 2. Youden-diagram of the results obtained for both dilutions. The two outliers are indicated by open circles. The mean values of participants= 300.4 μL⁻¹ and= 302.9 μL⁻¹ are plotted as vertical and horizontal lines, respectively. The diagonal with unity slope was inserted to illustrate that the results obtained from both dilutions are consistent with a linear relation within the uncertainties reached. The middle dashed square indicates boundaries corresponding to ±15% deviation from the respective mean values. If the outliers are disregarded, 2 out of 21 values are outside of this range, that is, 90% of the participants' results are within a limit of ±15%.